Theoretical and photophysical investigation of biologically active fluorophore: 2-amino-6-chlorofluoren-9-one

The optimized geometric parameters of the 2-Amino-6-chlorofluoren-9-one (2A6CF9O) compound were estimated by employing density functional theory. The electronic characteristics of the molecule were explored using molecular frontier orbital energies and the MEP surface. Kamlet's and Catalan's multiple linear regression techniques along with different polarity functions were used to investigate the influence of pure solvents on spectral properties. In the system, both general solute-solvent and hydrogen bonding interactions are active. However, as compared to normal solute-solvent interactions, hydrogen bonding interactions have a smaller role. In addition, using computed ground state dipole moment, solvatochromic correlations were employed to infer excited state dipole moment.     

A flow-through microfluidic chip for continuous dielectrophoretic separation of viable and non-viable human T-cells

Effective methods for rapid sorting of cells according to their viability are critical in T cells based therapies to prevent any risk to patients. In this context, we present a novel microfluidic device that continuously separates viable and non-viable T-cells according to their dielectric properties. A dielectrophoresis (DEP) force is generated by an array of castellated microelectrodes embedded into a microfluidic channel with a single inlet and two outlets; cells subjected to positive DEP forces are drawn toward the electrodes array and leave from the top outlet, those subjected to negative DEP forces are repelled away from the electrodes and leave from the bottom outlet. Computational fluid dynamics is used to predict the device separation efficacy, according to the applied alternative current (AC) frequency, at which the cells move from/to a negative/positive DEP region and the ionic strength of the suspension medium. The model is used to support the design of the operational conditions, confirming a separation efficiency, in terms of purity, of 96% under an applied AC frequency of 1.5 × 10⁶ Hz and a flow rate of 20 μl/h. This work represents the first example of effective continuous sorting of viable and non-viable human T-cells in a single-inlet microfluidic chip, paving the way for lab-on-a-chip applications at the point of need.     

Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review

Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic purposes. Affinity capillary electrophoresis (ACE) is one technique that can be used to examine the binding between drugs and serum proteins, or other agents found in serum or blood. This article will review the basic principles of ACE, along with related affinity-based capillary electrophoresis (CE) methods, and examine recent developments that have occurred in this field as related to the characterization of drug–protein interactions. An overview will be given of the various formats that can be used in ACE and CE for such work, including the relative advantages or weaknesses of each approach. Various applications of ACE and affinity-based CE methods for the analysis of drug interactions with serum proteins and other binding agents will also be presented. Applications of ACE and related techniques that will be discussed include drug interaction studies with serum agents, chiral drug separations employing serum proteins, and the use of CE in hybrid methods to characterize drug binding with serum proteins.     

A Happy Get-Together – Probing Electrochemical Interfaces by Non-Linear Vibrational Spectroscopy

Electrochemical interfaces are key structures in energy storage and catalysis. Hence, a molecular understanding of the active sites at these interfaces, their solvation, the structure of adsorbates, and the formation of solid-electrolyte interfaces are crucial for an in-depth mechanistic understanding of their function. Vibrational sum-frequency generation (VSFG) spectroscopy has emerged as an operando spectroscopic technique to monitor complex electrochemical interfaces due to its intrinsic interface sensitivity and chemical specificity. Thus, this review discusses the happy get-together between VSFG spectroscopy and electrochemical interfaces. Methodological approaches for answering core issues associated with the behavior of adsorbates on electrodes, the structure of solvent adlayers, the transient formation of reaction intermediates, and the emergence of solid electrolyte interphase in battery research are assessed to provide a critical inventory of highly promising avenues to bring optical spectroscopy to use in modern material research in energy conversion and storage.     

Experiments on crack identification in cantilever beams

In this paper we present an experimental investigation of the identification of crack location and size. By providing the first three natural frequencies through vibration measurements, curves of crack equivalent stiffness versus crack location are plotted, and the intersection of the three curves predicts the crack location and size. In the experiments, the cracked specimens were made using a wire-cut electrical discharge machine, and the cantilever beams were excited next to the free end by means of an impulse force hammer. In order to obtain the accurate natural frequencies from the transient signal measured, the method of zoom fast Fourier transform is adopted to improve frequency resolution. From experimental results, it is observed that the identification errors of crack location and size are less than 2% and 4%, respectively. The effectiveness of crack identification through vibration measurements is verified.     

DYNAMIC ANALYSIS OF A LOADED SHOULDER-POLE DURING WALKING

建立了扁担挑物的动力学模型和动力学方程,并进行了数值求解。扁担挑物时存在三种频率,即重物类似于单摆的振动频率、挑运者的步行频率和扁担-重物系统铅垂方向的振动频率。计算结果表明,为了获得较好的挑担体验,三种频率应该相互协调。当人挑担行走的频率为扁担-重物系统铅垂方向振动频率的70%,$\sim$,80%时,肩部铅垂附加动反力为重物重力的30%左右且与肩部铅垂运动同相,当重物类似于单摆的振动频率为人步行频率的25%,$\sim$,30%时,重物的摆角和肩部受到的水平附加动反力均较小,从而有利于人挑担行走。     

应用相位移法的二维光栅测量

本文介绍了二种二维位移测量的光栅方法。测试系统采用CCD摄象系统记录正交光栅图象,通过调整摄象机变焦镜头的放大倍数来控制采样的空间频率。采样后用数字信号处理的方法,通过软件手段实现水平向和垂直向光栅信号的分离,然后运用了相位移技术及载波—相位移技术得到二维位移。所开发的测试系统除具有高精度、高灵敏度的特点外,还具有较高的空间分辨率。     

传递矩阵－多项式法的改进及其应用

本文提出一种计算转子－滑动轴承－挤压油膜阻尼器转子系统的阻尼固有频率及稳定性的方法，即将传递矩奉多项式法改进后，应用于这类具有分叉结点的系统，直接得到系统的特征多项式，然后用Ｂａｉｒｓｔｏｗ－Ｎｅｗｔｏｎ僻因子法求出全部特征根。本方法具有占内存少、计算速度快、数值稳定等特点，文中对一转子模型进行了计算，其结果与实测基本相符。     

正交正放类网架结构固有频率及屈曲分析的样条Galerkin法

本文根据考虑剪切变形的具有３个广义位移的平板弯曲理论，把正交正放类平板网架假设为由三层不同性质材料组成的各向正交异性夹层板。文中以双５次Ｂ样条函数中的一组基底作为试函数，用加权残值法中的离散型Ｇａｌｅｒｋｉｎ法，导出了这类网架的频率方程和屈曲方程。数值算例表明，这种方法是方便而精确的。     

A GEOMETRIC THEORY IN INVESTIGATION ON MODE LOCALIZATION AND FREQUENCY LOCI VEERING PHENOMENA

According to the mapping theory in complex plane,the geometric features of eigen fre-quency loci of systems undergoing free vibrations are investigated.It is concluded that the phenomenaof curve coalescence and veering can be described in a unified manner from the singularities of map-ping from the complex parameter plane onto the complex frequency plane.The formation of a branchpoint in the parameter space is the foundation of explaining localization and veering phenornena.Bythe use of condensation to reduce the dimension of a system,the scope of application of the geometrictheory is widely expanded.The theory is applied to examples to verify the validity of the proposed ap-proach.The present work is an improvement and extension of recent work by M.S.Traintafyllou etal.,